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However, we were able to detect rifampicin-resistant colonies after scaling up the system to 1 L flasks and plating up to 1010 cells following treatment with both 3 µg/ml kanamycin and thiourea (data not shown). (attribution: Copyright Rice University, OpenStax, under CC BY 4.0 license) Beneficence is a principle that requires nurses to “do good” to actively promote their clients’ health and well-being. Thus, the loss of cellular potency associated with this substitution (over 100-fold loss in con1 replicon) and its ultimate appearance in patients who experience virologic rebound during danoprevir monotherapy reflects an increased rate of danoprevir dissociation compared to that observed with wild-type protein. In this method, the transducer emits a fundamental frequency with certain intensity (usually stronger than the one used in baseline IVUS) that stimulates the microbubbles to resonate at frequencies different from the frequencies to which it was exposed

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